Real-Time PCR (qPCR)

Real-time polymerase chain reaction (real-time PCR), or quantitative PCR (qPCR), is a versatile technique that is used to amplify and simultaneously detect and quantify target nucleic acid sequences. 

The StepOnePlus™ real-time PCR system from Applied Biosystems is a 96-well real-time PCR instrument perfect for both first-time and experienced users. With with intuitive data analysis and instrument control software, this system is designed to deliver precise, quantitative real-time PCR results for a variety of genomic research applications.

For messenger RNA detection, reverse transcription is required prior to qPCR. The Veriti® 96-Well Thermal Cycler from Applied Biosystems is a versatile instrument that provides such feature. In addition, this thermal cycler allows for fast RT-PCR for users who are unable to perform qPCR on their samples.

Applications

• Gene expression analysis
• Protein expression
• MicroRNA expression
• SNP genotyping
• Viral load analysis
• Translocation analysis

Specifications and features for StepOnePlus™ real-time PCR system

• For real-time PCR (qPCR) purposes
• Format: 1 x 96-well plate, 96 x 0.1 mL tubes, 12 x 8-tube strips
• Reaction volume: 10-30 µL
• Temperature range: 4-100 °C
• Run time: < 2 hrs/run in standard mode, < 40 min/run in fast mode
• Calibrated dye: VIC®, TAMRA™, NED, ROX™, SYBR® Green I, FAM™, JOE™
• Optics: 4 emission filters, Photodiode, Blue LED excitation source

Specifications and features for Veriti® 96-Well Thermal Cycler

• For RT-PCR or cDNA synthesis purposes
• Format: 1 x 96-well plate, 96 x 0.2 mL tubes, 12 x 8-tube strips
• Reaction volume: 10-80 µL
• Temperature range: 4-99.9 °C
• Touch screen